Therefore our results demonstrated that nuclear lamin b is a substantial component of the nuclear envelope in neutrophils similar to other cell types goldberg et al 2008. Taken together the levels of nuclear lamin b expression affect extracellular trap formation in a negative dose dependent manner. Humans have one gene lmna encoding a type lamins and two genes encoding b type lamins.
The nucleoskeleton includes the nuclear intermediate filaments formed by independent networks of a and b type lamins. The nuclear envelope ne embraces the genome with a rich tapestry of outer and inner membrane proteins some of which mechanically link the cytoskeleton to the nucleoskeleton 1. Decreasing lamin b phosphorylation by pkcα inhibition genetic deletion or by mutating the pkcα consensus sites on lamin b attenuates extracellular trap formation.
Furthermore we demonstrate that nuclear translocation of pkcα which serves as the kinase to induce lamin b phosphorylation and disassembly results in nuclear envelope rupture. Mutations in lamin genes can result in several genetic laminopathies which may be life threatening. Lamin proteins are involved in the disassembling and reforming of the nuclear envelope during mitosis the positioning of nuclear pores and programmed cell death.
The nuclear envelope fragments into membrane vesicles and the lamin filaments are disassembled into lamin dimers. Disassembly of the nuclear envelope and lamina occurs at prometaphase a period of the mitotic cell cycle that precedes chromosome segregation. Nuclear envelope assembly after mitosis.
The lamins are type v intermediate filaments which can be categorized as either a type lamin a c or b type lamin b 1 b 2 according to homology of their dna sequences biochemical properties and cellular localization during the cell cycle. The nuclear lamina consists of two components lamins and nuclear lamin associated membrane proteins.
