We also showed that the nuclear export of nsp1α was necessary for its ability for type i ifn inhibition. Nsp1α was shuttling between the nucleus and cytoplasm. Nsp1α was found to contain the classical nuclear export signal nes and nsp1α nuclear export was crm 1 mediated.
Wen et al 1995. The regulated export of many proteins from the nucleus is dependent on the presence of a nuclear export signal nes consisting of a leucine rich stretch of amino acids fischer et al 1995. In this report we demonstrate that stat1 can be dephosphorylated in the nucleus and actively exported.
The discovery of nuclear export signals ness in a number of proteins revealed the occurrence of signal dependent transport of proteins from the nucleus to the cytoplasm1 2 3 4 5 6 7 8 9 10 11 12. We analyzed the sequences and three dimensional structures of natural experimentally identified ness and of false positive ness that were generated from the database in order to identify properties that might distinguish the two groups of sequences. We compiled 200 nuclear export signal nes containing crm1 cargoes in a database named nesdb.
A nuclear export signal nes is a short target peptide containing 4 hydrophobic residues in a protein that targets it for export from the cell nucleus to the cytoplasm through the nuclear pore complex using nuclear transport it has the opposite effect of a nuclear localization signal which targets a protein located in the cytoplasm for import to the nucleus. The putative nls in exon 15 was initially thought to be the authentic nls but subsequent studies demonstrated that it fails to function. The nuclear localization signal nls in exon 1 the nuclear export signal nes in exon 3 and the caspase cleavage site amino acids 256 307 in exon 3 are labeled.
A nuclear export signal nes is a short target peptide containing 4 hydrophobic residues in a protein that targets it for export from the cell nucleus to the cytoplasm through the nuclear pore complex using nuclear transport it has the opposite effect of a nuclear localization signal which targets a protein located in the cytoplasm for import to the nucleus.
